IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cardiac mitochondrial function in acute endotoxemia: effect of (-)-epicatechin (EC)
Autor/es:
PEREYRA LEONARDO; VANASCO VIRGINIA; MARCHINI TIMOTEO; CESAR GUILLERMO FRAGA; GALLEANO MÓNICA; ALVAREZ SILVIA
Lugar:
Buenos Aires
Reunión:
Congreso; VIII International Congress of SFRBM South American Group; 2013
Institución organizadora:
Society for Free Radical Biology and Medicine
Resumen:
As mitochondrial dysfunction plays a vital role in the pathology of endotoxemia, the aim of this work was to evaluate the effects of dietary EC on cardiac mitochondrial damage. Rats (Sprague-Dawley, female, 180g) were fed on a diet enriched with EC (100 mg/kg bw/day) during 4 d. After this pretreatment, lipopolisaccharide (LPS, 10 mg/kg) was ip injected to produce acute endotoxemia. Experimental groups studied were: control (C), EC (E), LPS and LPS + EC (LPS + E). Studies were performed 6 h after LPS challenge in the heart. EC pretreatment prevented the increase observed, due to LPS challenge, in oxidized cardiolipin levels (40% increase, oxidative damage to lipid marker), and the decrease observed in complex I activity (20 % decrease, control value: 187 ± 22 umol/min. mg prot). EC did not prevent the LPS-mediated increase of NO-Hb blood levels, and the decrease in state 3 mitochondrial oxygen consumption, respiratory control ratio, and inner membrane potential. In summary, these results show that dietary EC ameliorates LPS-induced cardiac mitochondrial damage, by preserving a) essential mitochondrial lipids from oxidation and b) activity of key respiratory chain components.