Effect of dietary (-)-epicatechin on NO bioavailability in renal cortex of fructose-fed rats

PRINCE PD; ELESGARAY R; COSTA MA; VAZQUEZ-PRIETO MA; FRAGA CG; GALLEANO M

Buenos Aires

Congreso; VIII International Congress Society for Free Radical Biology and Medicine; 2013

Society for Free Radical Biology and Medicine, South American Group

Effect of dietary (-)-epicatechin on NO bioavailability in renal cortex of fructose-fed ratsPrince PD1, Elesgaray R2, Costa MA2, Vázquez-Prieto MA3, Fraga CG1 and Galleano M1 1Physical Chemistry (FFyB-UBA) and IBIMOL (UBA-CONICET) 2Physiology (FFyB-UBA) and IQUIMEFA (UBA-CONICET), Buenos Aires3Pathology, School of Medicine, UNCUYO and IMBECU-CONICET, Mendoza, ArgentinaMetabolic syndrome (MS) is associated with impaired renal function, being decreased nitric oxide (NO) bioavailability a relevant event. We evaluated the effect of (-)-epicatechin (EC) supplementation on NO bioavailability in the renal cortex of rats subjected to an experimental model of MS. Male Sprague-Dawley rats were divided into 3 groups: Control (C), Fructose (F) and Fructose + EC (FEC). Fructose was administered in the drinking water (10% w/v) and EC was supplemented in the diet (20mg/kg BW/d) for 8 w. NO synthase (NOS) activity and the expressions of inducible and endothelial NOS were evaluated. We found a significant increase in NOS activity in F and FEC respect to C. FEC did not show the increase in iNOS expression induced by fructose overload. eNOS expression was not affected by any treatment, but its activating phosphorylation at Ser1177 was significantly increased in FEC respect to F and C. Both NADPH dependent superoxide anion production and the expression of p47phox, a subunit of NADPH oxidase, were significantly higher in F respect to C and  FEC. These results suggest that EC improves NO bioavailability, stimulating its enzymatic production and decreasing superoxide production.UBACYT 20020100100659 and 20020100100060 and CONICET PIP- 11220110100612