Mitochondrial function in rat brain cortex and hippocampus after long term hypobaric hypoxia

CZERNICZYINIEC, A; LORES ARNAIZ, S; BUSTAMANTE, J; LA PADULA, P; COSTA, LE

Buenos Aires

Congreso; VIII International Congress Society for Free Radical Biology and Medicine; 2013

Society for Free Radical Biology and Medicine

Mitochondria isolated from hippocampus of rats submitted to hypobaric hypoxia for 1 month showed an increase in nitric oxide synthase along with a decrease in O2 consumption. In the present study mitochondrial function in brain of rats submitted to prolonged hypoxia was analyzed. Mitochondrial fraction was isolated from brain cortex and hippocampus of rats exposed to 53.8 kPa in a hipopressure chamber for 7 months (HH), and their controls (C) at 101.3 kPa. Nitric oxide production, determined by the spectrophotometric method of the oxyhemoglobin and O2 consumption, measured by a high resolution respirometer, were unchanged. Membrane depolarization, evaluated by flow citometry, increased (%) from 13.7 ± 1.2 to 25.6 ± 2.5 (p