Adipose tissue modifications during endotoxemia: modulation by dietary (-)-epicatechin

GEREZ EM; L FISHERMAN; PD PRINCE; MC LITTERIO; M GALLEANO; CG FRAGA

Buenos Aires

Congreso; VI International Conference on Polyphenols and Health; 2013

IBIMOL-CONICET, Facultad de Farmacia y Bioquimica, UBA

Adipose tissue is a key player in inflammation and its dysregulation is responsible for deleterious effects on several organs. In this work we evaluated the effects of dietary (-)-epicatechin (E) on parameters associated to global and local nitric oxide (NO) production and oxidative modifications in white adipose epididymal tissue (EWAT) in rats subjected to endotoxemia. Male Sprague-Dawley rats were fed on a diet enriched with E (ca. 100 mg/kg bw) during 4 d. After this pretreatment, lipopolysaccharide (LPS, 4 mg/kg) was ip injected to produce acute endotoxemia and animals were sacrificed 6 h after LPS administration. The 4 experimental groups were: Control (C), Control+E (CE), LPS (L) and LPS + E (LE). The increase in NO level in blood after LPS-challenge, evaluated by electronic paramagnetic resonance, was significantly higher in LE than in L. In EWAT from LE there was significant attenuation in the increases observed in TBARS and NADPH-dependent SOD inhibitable superoxide anion production in L. The same E effect was found in inducible-NO synthase expression. These results suggest that dietary E should be able to attenuate the EWAT dysregulation in terms of oxidative metabolism and iNOS induction during the onset of acute endotoxemia.