Systemic inflammatory response and oxidative stress following an acute exposure to environmental particulate matter

MARCHINI, TIMOTEO; MAGNANI, NATALIA; PAZ, MARIELA; TASAT, DEBORAH; GONZÁLEZ MAGLIO, DANIEL; ALVAREZ, SILVIA; EVELSON, PABLO

Buenos Aires

Congreso; VIII Meeting of the Society for Free Radical Biology and Medicine - South American Group; 2013

Society for Free Radical Biology and Medicine

The exposure to environmental particulate matter (PM) is associated with increased morbidity and mortality rates due to cardiopulmonary diseases. In this context, a systemic inflammatory response and oxidative stress are suggested to play a predominant role. The aim of this work was to evaluate this hypothesis, in an in vivo animal model of acute exposure to PM. Mice were intranasally instilled with Residual Oil Fly Ashes (ROFA) (1 mg/kg). Plasmatic cytokine profile, TBARS and carbonyl content, GSH and GSSG levels, ascorbic acid content, and SOD activity were evaluated 3 h after the exposure. TBARS and carbonyl content were significantly increased by 19% and 12%, respectively, in comparison with the control group. In ROFA-exposed mice, GSH was decreased by 27% while GSSG was increased by 42%, leading to a 50 % decrease in GSH/GSSG ratio (control 10.6). Ascorbic acid content and SOD activity were significantly decreased by 29% and 38%, respectively, in ROFA-exposed mice. This scenario was accompanied by a significant increase in plasmatic levels of proinflammatory cytokines (TNF-α and IL-6) after the ROFA exposure. The present findings may provide new evidence on the mechanism by which an acute exposure to environmental PM leads to adverse health effects.