Lung O2 metabolism after an acute exposure to transition metals present in ambient particulate matter

MAGNANI, NATALIA; MARCHINI, TIMOTEO; MELE, FLAVIA; MEBERT, ANDREA; DESIMONE, MARTIN; DIAZ, LUIS; ALVAREZ, SILVIA; EVELSON, PABLO

Buenos Aires

Congreso; VIII Meeting of the Society for Free Radical Biology and Medicine - South American Group; 2013

Society for Free Radical Biology and Medicine

Transition metals could play an important role in lung diseases mechanisms triggered by the exposure to ambient particulate matter (PM). The aim of this work was to study lung O2 metabolism after the exposure to transition metals-doped nanoparticles (NP). Swiss mice were intranasally instilled with silica NP containing Cd, Fe or Ni (0.01; 0.05; 0.10; 1.00 mg metal/kg body weight). NP were built by Stöber method, their aerodynamic diameter was assessed by dynamic light scattering and the metal content by atomic absorption. Tissue O2 consumption, NADPH oxidase (Nox) activity and TBARS content were evaluated in lung samples 1 h after instillation. No changes were observed after Cd-NP exposure in any dose or parameter evaluated. Fe-NP showed significantly increased O2 consumption, Nox activity and TBARS content at higher doses (0.01; 1,00 mg Fe/kg body weight). Regarding Ni-NP exposure, tissue O2 consumption, Nox activity and TBARS were found significantly increased in every concentration assessed. Transition metals like Fe and Ni seem to be important components involved in the altered lung oxidative metabolism after PM exposure. These findings provide new insights to the understanding of lung oxidative damage mechanisms triggered by metals present in environmental PM.