IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Evaluation of the antioxidant capacity in biological systems by electrochemical techniques.
Autor/es:
ALMALECK, HUGO; MUSACCO SEBIO, ROSARIO; SAPORITO MAGRIÑÁ, CHRISTIAN; SEMPRINE, JIMENA; GORDILLO, GABRIEL; BOVERIS, ALBERTO; REPETTO, MARISA
Lugar:
Buenos Aires
Reunión:
Congreso; VIII International Congress. Society for Free Radical Biology and Medicine.; 2013
Institución organizadora:
Society for Free Radical Biology and Medicine
Resumen:
The aim of this work is to analyze and compare responses of three electrochemical techniques in order to determine the antioxidant capacity of samples obtained from animal tissues where oxidative damage is caused by metal accumulation. These techniques are cyclic voltammetry (CV), cronoamperometry (CA) and square wave voltammetry (SWV). The results are obtained from analysis of the current waveform recorded when a programmed oxidative potential is applied to the working electrode immersed in an electrochemical cell containing the sample. The total antioxidant capacity of the sample is a function combining the biological oxidation potential and the intensity of the anodic current, reflecting the last one the concentration of the components. The electrical charge (the area under the wave) usually correlates with the concentration. In the case of CV and SWV, the existence of more than one peak is indicative of the presence of more than one species. On the other hand the lower is the oxidation peak potential the better is the antioxidant ability. Trolox was chosen as a reference antioxidant and the standard addition method was employed in order to determine antioxidants concentrations. The results are mutually consistent and demonstrate the utility of these techniques for the determination required.