IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
VMP1 is a novel inducible gene which activation is a switch for autophagy
Autor/es:
LO RE A; VACCARO, MI
Lugar:
San Diego
Reunión:
Congreso; Experimental Biology 2012; 2012
Institución organizadora:
ASBMB
Resumen:
<!--
/* Font Definitions */
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;
mso-font-charset:0;
mso-generic-font-family:auto;
mso-font-pitch:variable;
mso-font-signature:-520092929 1073786111 9 0 415 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{mso-style-unhide:no;
mso-style-qformat:yes;
mso-style-parent:"";
margin-top:0cm;
margin-right:0cm;
margin-bottom:10.0pt;
margin-left:0cm;
line-height:115%;
mso-pagination:widow-orphan;
font-size:11.0pt;
font-family:Calibri;
mso-ascii-font-family:Calibri;
mso-ascii-theme-font:minor-latin;
mso-fareast-font-family:Calibri;
mso-fareast-theme-font:minor-latin;
mso-hansi-font-family:Calibri;
mso-hansi-theme-font:minor-latin;
mso-bidi-font-family:"Times New Roman";
mso-bidi-theme-font:minor-bidi;
mso-ansi-language:ES-AR;}
.MsoChpDefault
{mso-style-type:export-only;
mso-default-props:yes;
font-size:11.0pt;
mso-ansi-font-size:11.0pt;
mso-bidi-font-size:11.0pt;
font-family:Calibri;
mso-ascii-font-family:Calibri;
mso-ascii-theme-font:minor-latin;
mso-fareast-font-family:Calibri;
mso-fareast-theme-font:minor-latin;
mso-hansi-font-family:Calibri;
mso-hansi-theme-font:minor-latin;
mso-bidi-font-family:"Times New Roman";
mso-bidi-theme-font:minor-bidi;
mso-ansi-language:ES-AR;}
.MsoPapDefault
{mso-style-type:export-only;
margin-bottom:10.0pt;
line-height:115%;}
@page WordSection1
{size:612.0pt 792.0pt;
margin:70.85pt 3.0cm 70.85pt 3.0cm;
mso-header-margin:35.4pt;
mso-footer-margin:35.4pt;
mso-paper-source:0;}
div.WordSection1
{page:WordSection1;}
-->
VMP1 is a novel inducible gene which activation is a
switch for autophagy
Andrea Lo Re, Maria Ines Molejon, Alejandro Ropolo,
Veronica Boggio, María I. Vaccaro
Autophagy is an
evolutionarily preserved degradation process of cytoplasmic cellular
constituents, which participates in cell homeostasis as well as in response to
disease. Autophagy acts in a myriad of cellular events leading to survival or cell death. The aim of this work was to study the
effector molecules that regulate the transcription of the inducible gene vmp1,
which expression triggers autophagy. Using a combination of luciferase assays,
expression analysis and shRNA strategies, we report the identification of the
promoter region of the human vmp1 gene and the molecular pathways that lead to
its activation. We show that vmp1 promoter responds to autophagy stimuli
through mTOR-dependent and mTOR-independent pathways. Besides we present the
essential sequence required for this activation containing the TATA Box and
several consensus binding sites for transcription factors related to the
cellular stress such as CREB, E2F1, c-Jun and c-Fos. We demonstrate that
overexpression of any of this factors induces VMP1 expression. Moreover using shRNA technology, p62 degradation and LC3
cleavage or recruitment we show that these
factors induce VMP1-mediated autophagy. Finally we found that p300 acts and is
required as a coactivator for VMP1 activation and autophagy. Our results define VMP1 as a target for cellular
stress mediators, which activation is a switch for autophagy. AL:CONICET;MIV:UBA-
CONICET- ANPCyT.