MITOCHONDRIAL PATHWAYS IN ENDOTOXEMIA: BIOENERGETICS AND ROS PRODUCTION IN H9c2 CARDIOMYOCYTES

VANASCO, VIRGINIA; ADÁN AREÁN, JUAN SANTIAGO; ALVAREZ, SILVIA; PAPPALETTERA, BRUNO HERNAN

Mar del Plata

Congreso; Reunión Anual de la Sociedad Argentina de Investigaciones Clínicas N°LXVII; 2023

Sociedad Argentina de Investigaciones Clínicas

Alterations in energy production, redox balance and active mitochondrialdynamics in cardiac tissue during endotoxemia have beenpreviously demonstrated by our group. The aim was to elucidate therole of mitochondria in energy and ROS production during endotoxemiain a cardiomyocytes cell model. Rat myoblast cell line H9c2was cultured under standard conditions. Myoblasts were differentiatedinto cardiomyocytes with 100 nM retinoic acid and treated for1, 3, or 6 h with serum from animals with severe endotoxemia (SE,8 mg kg-1 LPS) or low-grade endotoxemia (LE, 0.5 mg kg-1 LPS).ATP production and mitochondrial membrane potential were measuredto study mitochondrial function. ATP production was founddecreased by 38% at 1 h and 19% at 3 h in LE. Moreover, in SE thedecrease was 50% at 1 or 3 h and 84% at 6 h (control: 161 ± 17nmol ATP min-1 mg protein-1, p