Mitochondrial pathways in endotoxemia: bioenergetics and ROS production in H9c2 cardiomyocytes

PAPPALETTERA, BRUNO HERNAN; VANASCO, VIRGINIA; VICO, TAMARA ANTONELA; ALVAREZ, SILVIA; ADÁN AREÁN, JUAN SANTIAGO; HVOZDA ARANA, AILEN

Ciudad Autónoma de Buenos Aires

Workshop; REDOX BA 2023; 2023

Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires

Alterations in energy production, redox balance and active mitochondrial dynamics in cardiac tissue during endotoxemia have been previously demonstrated. The aim was to elucidate the role of mitochondria in energy and ROS production during endotoxemia in a cardiomyocytes cell model. Rat myoblast cell l ine H9c2 were differentiated into cardiomyocytes with 100 nMretinoic acid and treated for 1, 3, or 6 h with serum from animals with severe endotoxemia (SE, 8 mg kg 1 LPS) or low grade endotoxemia (LE, 0.5 mg kg 1 LPS). ATP production was found decreased by 38% at 1 h and 19% at 3 h in LE. Moreover, in SE the decrease was 50% at 1or 3 h and 84% at 6 h (control: 161±17 nmol ATP min 1 mg protein 1 , p