Exploring mitochondrial pathways in endotoxemia: bioenergetics and ROS production analysis in H9c2 cardiomyocytes

VICO, TAMARA; VANASCO, VIRGINIA; PAPPALETTERA, BRUNO HERNAN; EVELSON, PABLO; ADÁN AREÁN, JUAN SANTIAGO; MAGNANI, NATALIA; ALVAREZ, SILVIA

Conferencia; SFRBM-SFRRI 2023 Conference; 2023

Alterations in energy production, redox balance and active mitochondrial dynamics in cardiac tissue during endotoxemia have been previously demonstrated. The aim was to elucidate the role of mitochondria in energy and ROS production during endotoxemia in a cardiomyocytes cell model. Rat myoblast cell line H9c2 were differentiated into cardiomyocytes with 100 nM retinoic acid and treated for 1, 3, or 6h with serum from animals with severe endotoxemia (SE, 8 mg kg-1 LPS) or low-grade endotoxemia (LE, 0.5 mg kg-1 LPS). ATP production was found decreased by 38% at 1 h and 19% at 3 h in LE. Moreover, in SE the decrease was 50% at 1 or 3 h and 84% at 6 h (control: 161±17 nmol ATP min-1 mg protein-1, p