Pre-ischemic vagus nerve stimulation improves long term left ventricular function and reduces fibrosis in ischemic hearts with or without reperfusion

MENDEZ DIODATI N; BARBIERI I; MORALES C; BUCHHOLZ B; FRANCO RIVEROS VB; GONZÁLEZ PEÑA B; CASANOVA V; GELPI RJ; BERNATENE E; CICALE E; DONATO M

Mar del Plata. Buenos Aires

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Investigación de Clínica; 2022

Sociedad Argentina de Investigación de Clínica ? SAIC

PRE-ISCHEMIC VAGUS NERVE STIMULATION IMPROVES LONG-TERM LEFT VENTRICULAR FUNCTION AND REDUCES FIBROSIS IN ISCHEMIC HEARTS WITH OR WITHOUT REPERFUSIONVerena B. Franco-Riveros1,2, Nahuel Méndez Diodati1,2, Eduardo Bernatené1,2, Beatriz González Peña1, Ignacio Barbieri1, Eliana Cicale3, Verónica Casanova3, María Celina Morales1, Martín Donato1,2, Ricardo J. Gelpi1,2, Bruno Buchholz 1,21 Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología. Instituto de Fisiopatología Cardiovascular. 2 Consejo Nacional de Investigaciones Científicas y Técnicas ? CONICET. 3 Universidad de Buenos Aires, Facultad de Veterinaria.The aim was to determine the effects of brief electric vagus nerve stimulation (VS) applied before ischemia on acute myocardial infarction (MI) and its long-term benefits on an experimental ischemia-reperfusion and non-reperfusion model. Mice underwent regional MI for 45min, followed by either 2 hours (2hR) or 28 days of reperfusion (28dR), with or without 10min of pre-ischemic VS. LVF was assessed by catheterization through the right carotid artery and, by echocardiography: EF, SF, IVRT. Participation of muscarinic receptors (MR) was determined by the administration of atropine (ATR) during VS. Infarct size (IS) was measured by TTC stain on acute groups. Histological measurements were also assessed to study myocardial IS and remodeling. VS decreased IS after 2hR, from 65.3±1.7% to 43±2.1% (p