Voluntary wheel running and Trx1-overexpression in mice subjected to ischemia/reperfusion injury.

OSSANI, GEORGINA; LEE, AURORA; GIMENEZ, SOFÍA; D´ANNUNZIO, VERÓNICA; PEREZ, VIRGINIA; GODOY, EUGENIA; LEE, BRIAN; BUCHHOLZ, BRUNO; FRANCO RIVEROS, VERENA; YANAJE, YOHANA; GRECO, CARLA; GELPI, RICARDO J

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2022

Voluntary wheel running and thioredoxin-1 (Trx1) overexpression reduce myocardial injury caused by acute ischemia/reperfusion (I/R). However, Trx1 role in exercise is not fully understood. The aim was to study if Trx1is involved in the exercise cardioprotection mechanism. Wild type mice hearts (Wt), transgenic mice hearts overexpressing Trx1, and a dominant negative mutant (DN) of Trx1 were used. Mice were divided in exercise group (E) and sedentary group (S). Mice were placed in cages fitted with running wheels for 4weeks. After the exercise-training period,mice hearts were subjected to 30min of I and 120min of R (Langendorff technique). The assessment of the infarct size was performed using TTC. Moreover, heart,quadriceps, gastrocnemius,and soleus weightwere measured. Besides, body weight, caloric intake and running distance were masured. Data were expressed as mean±SEM and p