Cardioprotective effect of remote ischemic preconditioning through attenuation of oxidative stress and inflammatory response

GARCÉS M; GELPI RJ; BIN E; DONATO M; MAGNANI N; EVELSON P

Siena

Congreso; The Future of Redox Biology. Siena 2022; 2022

SFRR-I

Cardiac remote ischemic preconditioning (rIPC) is a treatment to protect the heart against an ischemia and reperfusion-induced injury such as myocardial infarction (MI). Our aim was to evaluate if rIPC attenuated the cardiac function alterations on MI focusing on systemic oxidative stress and inflammation as possible mechanisms mediating these effects. Rats were anesthetized and MI was induced by global ischemia through coronary ligation. In a second group, a rIPC protocol was performed while a control group without treatments was added. Cardiac function parameters, LVEDA and LVESA, showed an increase in MI group while the ejection fraction was significantly lower. rIPC strategy showed attenuation of these alterations while increased circulating levels of MMP-9 and CK were decreased compared to MI group. Plasmatic antioxidant redox status showed increased SOD activity and higher GSSG levels on MI group. Moreover, 4-HNE expression and carbonyl content were increased. Systemic inflammatory response triggered by MI was increased as shown by TNF-α levels. rIPC prevented the changes induced by MI. These results provide direct evidence linking both, systemic inflammation and oxidative stress, and altered cardiac function following MI and contribute to the understanding of rIPC as a non-pharmacological treatment in cardiovascular disease.