Oxidative effects of in vivo exposure to Fe in marine invertebrates. Simposio Metals: oxidative stress relationship in biological systems.

PUNTARULO S

Salta

Congreso; XXXIX Anual Meeting of the Argentinean Biophysical Society 2010. Workshop CeBEM-Structural Biology in Latin America.; 2010

SAB

The effect of in vivo Fe exposure on the oxidative metabolism of the bivalve M. arenaria was studied. Fe, supplemented in natural seawater, resulted in a significant increase in the total Fe content in the bivalve digestive gland (DG) between 9 to 17 days. The generation of reactive oxygen species and ascorbyl radical content were affected following a biphasic profile showing significant increases by day 2 and 9 of Fe exposure. The labile Fe pool was elevated over control values by day 7, and maintained this increase until day 17. The content of NO, assessed by EPR spin trapping, was 60% lower in DG of animals exposed for 2 days to Fe than in control values, with no further changes. This biphasic profile suggested that at early stages of Fe supplementation the cellular control mechanisms were operative to limit oxidative damage, but further Fe exposure overwhelmed these abilities. Moreover, the second phase could be understood as the consequence of the exhaustion of cellular protective systems, that could also involve NO. Comparatively, Fe toxicity in these animals was higher as compared to Fe effect in naturally exposed L. elliptica to similar Fe concentrations, suggesting adaptation features to the environmental conditions.