Cellular Fe in Fetal Brain gamma-Irradiated Both in vivo and in vitro.

ROBELLO E., PILONI N. Y PUNTARULO S.

Salta

Congreso; XXXIX Anual Meeting of the Argentinean Biophysical Society 2010. Workshop CeBEM-Structural Biology in Latin America. 3rd La tin American Protein Society Meeting; 2010

SAB

This study was aimed to test comparatively the effect of gamma-irradiation on Fe content in the fetal brain irradiated in vitro and in vivo. In vivo irradiation was performed by exposure of the mother to g-irradiation (2 G) at 17th gestational days (GD) and cells of rat brains were kept on culture for 0, 1, 2 and 4 h post-irradiation (pi). In vitro irradiation (2 G) was performed exposing isolated fetal brain cells taking from foetuses after 17 GD. In the in vivo model there were a significant increase in the total Fe content, assessed spectrophotometrically, at 4 h pi (4.2±0.1, 5.2±0.3, 5.1±0.8 and 14±1 nmol/106 cells for 0, 1, 2 and 4 h pi). A significant reduction on the labile iron pool (LIP), assessed employing calcein, at 2 h pi (0.27±0.04 nmol/106 cells), was detected as compared to non-irradiated cells (0.39±0.02 nmol/106 cells). In the in vitro model, there were neither alterations on the total Fe content (3.2±0.7, 3.3±0.9, 4.0±0.2 and 3.5±0.3 nmol/106 cells at 0, 1, 2 y 4 h pi) nor on the LIP (0.42±0.03, 0.41±0.02, 0.4±0.1 and 0.51±0.03 nmol/106 cells at 0, 1, 2 y 4 h pi). These results suggest that alterations on Fe content in fetal brain cells depend on the complex interactions between the maternal and fetal plasma that can not be reproduced adequately by the in vitro model. Supported by grants from UBA and CONICET.