Bioenergetic profiling in the skin

CELA, ELIANA; PAZ, MARIELA; MARCHINI, TIMOTEO; LEONI, JULIANA; GONZALEZ MAGLIO, DANIEL; MARGNANI, NATALIA; EVELSON, PABLO

Clinical Bioenergetics

Elsevier

Lugar: Londres; Año: 2020; p. 285 - 308

AbstractThe skin, the most external organ of the body, is constituted by a variety of cells, including keratinocytes, fibroblasts, melanocytes, and a wide diversity of immune cells. After exposure to harmful stimuli, such as those produced by environmental radiation or air pollution, bioenergetics of skin cells may be affected. There are different methodological approaches to study these bioenergetic alterations, including in vitro models using cell lines that represent only an isolated target of the stimuli, in vitro 3D cultures that also include the terminal differentiation of the keratinocytes, and in vivo approaches using different animal models, in which complex interactions take place. This chapter aims to discuss different techniques useful to study bioenergetic alterations of skin cells, commenting benefits, and limitations of their use, also considering the usefulness of each skin model.