In vivo and in vitro chemiluminescence methods to evaluate the occurrence of oxidative stress in whole organs and tissue homogenates

LLESUY, SUSANA; FERREIRA, SANDRA; EVELSON, PABLO

Handbook of Chemiluminescent Methods in Oxidative Stress Assessment

Research Signpost

Lugar: Kerala, India; Año: 2008; p. 29 - 40

There is great interest in the determination of the occurrence of oxidative stress experienced by a tissue under a pathologic condition. Several methods have been developed in order to establish the participation of oxidants or the role of antioxidants in a certain pathology. In the present chapter we present a discussion of three different methods that help to evaluate if a given tissue is under an oxidative stress condition. The methodology includes in vivo organ chemiluminescence, hydroperoxide-initiated chemiluminescence and total antioxidant reactive potential (TRAP) of tissue homogenates.