IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
artículos
Título:
Espectrometría de masas en condiciones
Autor/es:
ALICIA B. POMILIO; EDUARDO BERNATENE; ARTURO A. VITALE
Revista:
ACTA BIOQUÃMICA CLÃNICA LATINOAMERICANA
Editorial:
FEDERACION BIOQUIMICA PROVINCIA BUENOS AIRES
Referencias:
Año: 2010 vol. 44
ISSN:
0325-2957
Resumen:
Ambient mass spectrometry is a useful and rapidly growing new field that provides high sensitivity
mass spectra directly from surfaces at atmospheric pressure. Various ionization
techniques, including desorption electrospray ionization (DESI), direct analysis in real time
(DART), plasma assisted desorption ionization (PADI) and extractive electrospray ionization
(EESI) have been used. This paper refers particularly to the fundamentals and applications
of DESI-MS based on imaging mass spectrometry. Among other applications, DESI is used for
direct analysis of drugs and pharmaceutical formulations, samples of biological fluids, forensics,
fingerprints, food, cultures of bacteria, identification and spatial distribution of
chemicals in animal and plant tissues, and molecular biomarkers. It highlights the possibility
of combination with thin layer chromatography and electropherograms to identify the
compounds by mass spectrometry. This technique requires no sample preparation, and does
not involve the use of matrix of ionization. It simplifies greatly the experimental procedure
and avoids the redistribution of analytes during matrix deposition. The forensic analysis carried
out by DESI-MS DESI-MS/MS is discussed, including the detection of explosives and
chemical warfare agents on solid surfaces near or at a distance from the mass spectrometer,
analysis of fabric or clothing for explosives and drugs, image analysis for verification of documents,
analysis of human skin, gunshot residue analysis, analysis of toxic gases and
industrial warfare agent simulants, petroleum distillates and synthetic polymers. Aplications
in the field of lipidomics, proteomics, and metabolomics are analyzed. Finally, current information
on the quantitative analysis performed by DESI-MS is provided.