Assessment of oxidative balance in hydrophilic cellular environment in Chlorella vulgaris exposed to glyphosate.

SUSANA PUNTARULO; GABRIELA MALANGA; JUAN MANUEL OSTERA

CHEMOSPHERE

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2020

0045-6535

The studied hypothesis is that glyphosate (GLY) can affect the oxidative balance in the hydrophilicintracellular medium in non-target Chlorella vulgaris cells. Analytical GLY (5 mM) and a commercialproduct (RUP) (5 mM) supplementation, did not affect the growth profile. Neither in latent (Lag) nor inexponential (Exp) phase of development, there were significant differences in the cellular abundance,evaluated as cell number, after the supplementation with GLY or RUP. The ascorbyl (A) content wassignificantly increased in the presence of GLY or RUP, in Lag and Exp phase of growth. No changes wereobserved in stationary (St) phase after supplementation with either GLY or RUP. Ascorbate (AH) contentwas decreased by 30% in Exp phase of development the presence of RUP. In St phase of the developmentboth, the administration of either GLY or RUP decreased the antioxidant content by 34 and 37%,respectively. The supplementation with GLY and RUP lead to a significant 5- and 10-fold increase in Expphase, respectively in the A/AH-content ratio, assessed as a damage/protection ratio in the hydrophilicfraction of the cells, as compared to controls. Neither GLY nor RUP affected the ratio in cells in St phase ofdevelopment. The data presented here showed experimental evidence that suggested that oxidativebalance in the hydrophilic environment is affected by GLY, even at the low to medium concentrationscurrently used. The effect seems as reversible either because of the magnitude of the herbicidedependent damage or the antioxidant activity activated.