IBIMOL 23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
artículos
Título:
Thioredoxin-1 is required for the cardioprotecive effect of sildenafil against ischemia/reperfusion injury and mitochondrial dysfunction in mice
Autor/es:
MAZO T; CONTI MI; GELPI RJ; ZAOBORNYJ T; GOMEZ A; D´ANNUNZIO V; PEREZ MV; TRIPODI V
Revista:
FREE RADICAL RESEARCH
Editorial:
TAYLOR & FRANCIS LTD
Referencias:
Lugar: Londres; Año: 2019 vol. 53 p. 993 - 1004
ISSN:
1071-5762
Resumen:
Sildenafil is a phosphodiesterase type 5 inhibitor which confers cardioprotection against myocardial ischemia/reperfusion (I/R) injury. The aim of this study was to determine if Trx1 participates in cardioprotection exerted by sildenafil in an acute model of I/R, and to evaluate mitochondrial bioenergetics and cellular redox status. Langendorff-perfused hearts from wild type mice (WT) and a dominant negative (DN-Trx1) mutant of Trx1 were assigned to placebo or sildenafil (0.7 mg/kg i.p.) and subjected to 30 min of ischemia followed by 120 min of reperfusion. WT + S showed a significantly reduction of infarct size (51.2±3.0% vs. 30±3.0%, P0.05) and in DN-Trx (35%, P
