IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
artículos
Título:
The possible role of microcystin (D-Leu1 MC-LR) as an antioxidant on Microcystis aeruginosa (Cyanophyceae). In vitro and in vivo evidence
Autor/es:
GIANNUZZI, L.; HERNANDO, M.; MALANGA, G.
Revista:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. TOXICOLOGY & PHARMACOLOGY
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Año: 2019 vol. 225 p. 1 - 9
ISSN:
1532-0456
Resumen:
Microcystins constitute a serious threat to the quality of drinking water worldwide. However, the eco-physiologicalrole of them is not completely known and it is suggested that toxins can play a role in the antioxidantprotection. The objective of this study was to evaluate the microcystin antioxidant capacity in vitro by ElectronicParamagnetic Resonance, highly specific for the different reactive oxygen species and in vivo by 7 days exposureof Microcystis aeruginosa to high (29 °C) temperature in addition to a 26 °C control condition. An effective in vitroantioxidant activity was observed for [D-Leu1]MC-LR against hydrosoluble radicals. As far as we know, this isthe first in vitro record of the role of MC as antioxidant. In addition, a significant increase in cellular biomass wasobserved under 26 °C in cultures with [D-Leu1]MC-LR supplementation in coincidence with a significant decreaseof reactive species. For cultures at 29 °C, the antioxidant role of toxins was inconclusive probably due tothe presence of different reactive species generated during the experiment. Thus, MC could scavenge certainreactive species associated with the antioxidant role of CAT or the %OH content by SOD activity (not measured)and then CAT activity could be lower in the presence of MC. Reinforcing our hypothesis, the [D-Leu1]MC-LRconsumption after 7 days was significantly higher in cells with [D-Leu1]MC-LR supplementation in both 26 °Cand 29 °C.When the production of reactive species was controlled by the scavenger activity of antioxidants plusMC, cells avoided the potential oxidative damage and started with exponential growth.