IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
artículos
Título:
Protective effects of the synthetic cannabinoids CP55,940 and JWH-015 on rat brain mitochondria upon paraquat exposure.
Autor/es:
VELEZ-PARDO, C.; JIMENEZ-DEL RIO, M.; LORES-ARNAIZ, S.; BUSTAMANTE, J.
Revista:
NEUROCHEMICAL RESEARCH
Editorial:
SPRINGER/PLENUM PUBLISHERS
Referencias:
Año: 2010 vol. 35 p. 1323 - 1332
ISSN:
0364-3190
Resumen:
Abstract The effects of cannabinoids in mitochondria
after acute oxidative stress insult are not fully established.
We investigated the ability of CP55,940 and JWH-015 to
scavenge reactive oxygen species and their effect on
mitochondria permeability transition (MPT) in either a
mitochondria-free superoxide anion generation system,
intact rat brain mitochondria or in sub-mitochondrial particles
(SMP) treated with paraquat (PQ). Oxygen consumption,
mitochondrial membrane potential (Dwm) and
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
after acute oxidative stress insult are not fully established.
We investigated the ability of CP55,940 and JWH-015 to
scavenge reactive oxygen species and their effect on
mitochondria permeability transition (MPT) in either a
mitochondria-free superoxide anion generation system,
intact rat brain mitochondria or in sub-mitochondrial particles
(SMP) treated with paraquat (PQ). Oxygen consumption,
mitochondrial membrane potential (Dwm) and
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
after acute oxidative stress insult are not fully established.
We investigated the ability of CP55,940 and JWH-015 to
scavenge reactive oxygen species and their effect on
mitochondria permeability transition (MPT) in either a
mitochondria-free superoxide anion generation system,
intact rat brain mitochondria or in sub-mitochondrial particles
(SMP) treated with paraquat (PQ). Oxygen consumption,
mitochondrial membrane potential (Dwm) and
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
The effects of cannabinoids in mitochondria
after acute oxidative stress insult are not fully established.
We investigated the ability of CP55,940 and JWH-015 to
scavenge reactive oxygen species and their effect on
mitochondria permeability transition (MPT) in either a
mitochondria-free superoxide anion generation system,
intact rat brain mitochondria or in sub-mitochondrial particles
(SMP) treated with paraquat (PQ). Oxygen consumption,
mitochondrial membrane potential (Dwm) and
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O2
Dwm) and
MPT were determined as parameters of mitochondrial
function. It is found that both cannabinoids effectively
attenuate mitochondrial damage against PQ-induced oxidative
stress by scavenging anion superoxide radical
(O22
-) and hydrogen peroxide (H2O2), maintaining Dwm-) and hydrogen peroxide (H2O2), maintaining Dwm
and by avoiding Ca2?-induced mitochondrial swelling.
Understanding the mechanistic action of cannabinoids on
mitochondria might provide new insights into more effective
therapeutic approaches for oxidative stress related
disorders.
Understanding the mechanistic action of cannabinoids on
mitochondria might provide new insights into more effective
therapeutic approaches for oxidative stress related
disorders.
Understanding the mechanistic action of cannabinoids on
mitochondria might provide new insights into more effective
therapeutic approaches for oxidative stress related
disorders.
2?-induced mitochondrial swelling.
Understanding the mechanistic action of cannabinoids on
mitochondria might provide new insights into more effective
therapeutic approaches for oxidative stress related
disorders.