Superoxide and hydrogen peroxide productions by NO-inhibited complex III

BOVERIS A; BOMBICINO SS; IGLESIAS DE; VALDEZ LB

Biocell

Corsino Impresores S.R.L.

Lugar: Mendoza; Año: 2016 vol. 40 p. 27 - 30

1667-5746

Complex III plays a central role in the mitochondrial respiratory chain transferring electrons from ubiquinol to cytochrome c and pumping protons to the intermembrane space, contributing to the protonmotive force. Furthermore, complex III can act as a source of O2- in the presence of ubiquinol and antimycin, which blocks the oxidation of the cytochrome b hemes. The O2?- dismutation catalyzed by superoxide dismutase produces H2O2, a known second messenger in redox signalling. Results from our laboratory have shown that NO, released from GSNO or from SPER NO or generated by mtNOS, inhibits electron transfer at ubiquinone-cytochrome b area producing antimycin-like effects. Thus, both antimycin- and NO-inhibited complex III showed a high content of cytochromes b in the reduced state (79 and 71%, respectively) and an enhancement in the ubisemiquinone EPR signal at g=1.99 (42 and 35%, respectively). As consequence, O2?- and H2O2 productions were increased, being the O2?-/H2O2 ratio equal to 1.98 in accordance with the stoichiometry of the O2?- disproportionation. The interruption of the oxidation of cytochromes b by NO leads to an enhancement of the steady-state concentration of UQH? allowing cytochrome bc1 complex to act as a source of reactive oxygen species in physiological conditions