IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
artículos
Título:
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
Autor/es:
KLIONSKY, DANIEL (AUTHOR Nº 1); ZUGHAIER, SUSU (AUTHOR Nº 1425); ALVAREZ, SILVIA (AUTHOR Nº 41)
Revista:
AUTOPHAGY
Editorial:
LANDES BIOSCIENCE
Referencias:
Lugar: Austin, Texas; Año: 2016 vol. 12 p. 1 - 222
ISSN:
1554-8627
Resumen:
Here, we present a set of guidelines for the selection andinterpretation of methods for use by investigators who aim toexamine macroautophagy and related processes, as well as forreviewers who need to provide realistic and reasonable critiquesof papers that are focused on these processes. These guidelinesare not meant to be a formulaic set of rules, because the appropriateassays depend in part on the question being asked andthe system being used. In addition, we emphasize that no individualassay is guaranteed to be the most appropriate one inevery situation, and we strongly recommend the use of multipleassays to monitor autophagy. Along these lines, because of thepotential for pleiotropic effects due to blocking autophagythrough genetic manipulation, it is imperative to target by geneknockout or RNA interference more than one autophagyrelatedprotein. In addition, some individual Atg proteins, orgroups of proteins, are involved in other cellular pathwaysimplying that not all Atg proteins can be used as a specificmarker for an autophagic process. In these guidelines, we considerthese various methods of assessing autophagy and whatinformation can, or cannot, be obtained from them. Finally, bydiscussing the merits and limits of particular assays, we hope toencourage technical innovation in the field.