IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
artículos
Título:
The Effect of Seasonality on Oxidative Metabolism in Nacella (Patinigera) magellanica.
Autor/es:
MALANGA G., ESTEVEZ M.S., CALVO J., ABELE D., PUNTARULO S.
Revista:
Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Referencias:
Año: 2007 vol. 146 p. 551 - 558
Resumen:
The aim of this work was to study the seasonal variation on the aerobic metabolism and the response of oxidative stress parameters in the digestive glands of the limpet Nacella (P.) magellanica. Sampling was carried out from July (winter) 2002 to July 2003 in the Beagle Channel, Tierra del Fuego, Argentina. Whole animal respiration rates increased in early spring as the animals spawned and remained elevated throughout summer and fall (winter: 0.09±0.02 mmol O2 h-1 g-1; summer: 0.31±0.06 mmol O2 h-1 g-1). Oxidative stress was assessed at the hydrophilic level as the ascorbyl radical content/ascorbate content ratio (A· /AH-). The A· /AH- ratio showed minimum values in winter (3.7±0.2 10-5 AU) and increased in summer time (18±5 10-5 AU). A similar pattern was observed for lipid radical content (122±29 pmol mg-1FW in winter and 314±45 pmol mg-1FW in summer), iron content (0.99±0.07 and 2.7±0.6 nmol mg-1FW in winter and summer, respectively) and catalase activity (2.9±0.2 and 7±1 U mg-1FW in winter and summer, respectively). Since nitrogen derived radicals are thought to be critically involved in oxidative metabolism in cells, nitric oxide content was measured and a significant difference in the content of the Fe–MGD–NO adduct in digestive glands from winter and summer animals was observed. Taken together, the data indicate that both oxygen and nitrogen radical generation rates in N. (P.) magellanica are strongly dependent on seasonal.