UNIDEF   23986
UNIDAD DE INVESTIGACION Y DESARROLLO ESTRATEGICO PARA LA DEFENSA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of Cuticular Hydrocarbons in Intra-specific Communication of Cimex lectularius (Cimicidae: Hemiptera)
Autor/es:
PABLO SANTO ORIHUELA; CÁCERES MARIANO; CLAUDIA VASSENA; PAULA V GONZALEZ
Lugar:
VALPARAISO
Reunión:
Congreso; 5th Congress of the Latin American Association of Chemical Ecology; 2018
Institución organizadora:
Pontificia Universidad Católica de Valparaíso
Resumen:
During the last two decades, the upsurge of bed bugs infestations and recurrent failure in chemical control practices were reported worldwide. The research efforts should be addressed to develop innovative control tools. In particular, a new approach based on chemical communication of bed bugs could improve an IMP program. In this study, we evaluated the role played by cuticular hydrocarbons compounds (CHC) of C. lectularius to mediate their aggregation/attractant behavior. We hypothesized that CHCs are involved in chemotactic recognition between bed bugs. For assessing the effect of cuticular extracts, groups of adults consisted of 8, 16, 32 and 64 individuals (male or female groups) were isolated for 6 days. Then, cold-euthanized groups were placed into vials and covered with dichloromethane (ranging 20 μl?4 ml, proportionally with a total mass of insects in each group). After 15 min of extraction, the solvent was evaporated and reconstituted with 40 μl of DCM and used for the stimuli test. The experimental arena consisted of a circle filter paper divided into two zones: the treatment zone was impregnated with cuticular extracts and solvent only in the control. First-stage nymph and virgin adult of both sexes were released individually and its activity was recorded during 15 minutes using a video-tracking equipment. The measurement of variables that included distance, velocity, and mobility was quantified with EthoVision XT 10.1 software. Results showed a dose-response effect to different extract groups. Whilst a positive preference was observed toward 16 and 32 groups, extracts in upper dose generated avoidance behavior. In all cases, there were no differences between the sexes. Currently, we are performing identification and quantification of chemicals compounds by GC-MS analysis to elucidate the specific compounds involved in their recognition and then evaluate the effectiveness of candidate blend its possible usage in strategies for monitoring bed bugs.