UNIDEF   23986
UNIDAD DE INVESTIGACION Y DESARROLLO ESTRATEGICO PARA LA DEFENSA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
OXIDATION OF ETHANOL TO ACETALDEHYDE AND OXIDATIVE STRESS COULD BE INVOLVED IN THE ALCOHOL DRINKING PROMOTED CELL INJURY OF THE RAT UTERUS
Autor/es:
F. FORMOSA LEMOINE; M.E. MACIEL; G.D. CASTRO; M.H. COSTANTINI
Lugar:
Goiania
Reunión:
Congreso; XX Congresso Brasileiro de Toxicologia; 2017
Institución organizadora:
Sociedade Brasileira de Toxicologia
Resumen:
It is known that alcohol drinking can lead to impairment in reproductive function in women. Controversial studies from others suggested that alcohol intake could be associated with some deleterious effects in the uterus. Not all the effects of alcohol drinking on female reproductive organs can be explained in terms of endocrine disturbances. Deleterious effect of alcohol or its metabolites in situ could also play a role. In this study we analyze the possibility that part of these effects were mediated through alterations of uterus function related to ethanol oxidation to acetaldehyde occurring in that tissue. We found a metabolism of alcohol to acetaldehyde in the rat uterus tissue cytosolic fraction mediated by xanthine oxidoreductase, requiring a purine cosubstrate and inhibited by allopurinol. This activity was detected by histochemistry in the epithelium and aldehyde dehydrogenase activity was detected in the muscular layer and in the serosa. There was also a microsomal process, not requiring NADPH and of enzymatic nature, oxygen-dependent and inhibited by diethyldithiocarbamate and diphenyleneiodonium. After alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uterus was observed, accompanied by significant alterations in its epithelial cells. Ultrastructure of uterus from rats treated with standard Lieber & De Carli liquid diet for 28 days revealed extensive vacuolization in cytoplasm and loss of cell content. In addition, we observed the promotion of oxidative stress as evidenced by increased response in the t-butylhydroperoxide induced chemiluminiscence and the depletion of the protein sulfhydryl content. Minor but significant depletion were observed in the GSH content, accompanied by a tendency to decrease in the GSH/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that in the rat uterus, metabolism of ethanol to acetaldehyde and oxidative stress may play a role in alcohol effects on female reproductive function.Acknowledgments: PIP 11220150100067CO (CONICET, Argentina)