UNIDEF   23986
UNIDAD DE INVESTIGACION Y DESARROLLO ESTRATEGICO PARA LA DEFENSA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Independent and joint action of the repellents DEET e IR3535 on Triatoma infestans
Autor/es:
CALCAGNO, J. A.; REYNOSO, M.M.N.; ALZOGARAY, R.A.; ZERBA, E.N.
Lugar:
Fos do Iguaçu
Reunión:
Congreso; 32nd Annual Meeting of the International Society of Chemical Ecology (ISCE) and the 4th Congress of the Latin American Association of Chemical Ecology (ALAEQ); 2016
Institución organizadora:
Universidade Federal do Paraná
Resumen:
N,N-diethyl-3-methylbenzamide (DEET) and ethyl 3-[acetyl(butyl)amino]propanoate (IR3535) are synthetic insect repellents used worldwide to protect people from bites of blood-sucking arthropods. Triatoma infestans (Hemiptera: Reduviidae) is the main vector of Chagas disease in Argentina and neighboring countries. The objective of this work was to evaluate the repellence produced by these substances, individually or mixed, on fifth instar nymphs of T. infestans. The experimental arena was a circle of filter paper (diam.: 11 cm). Half the disc was treated with a solution of repellent in acetone; the other half, with acetone alone. A nymph was gently placed on the middle of the arena, and its position in the next ten minutes was quantified using an image analyzer (Videomex V, Columbus). The following concentrations of DEET and IR3535 were studied: 0 (control), 0.38, 1.15, 3.5, 10.4 and 31.0 µg/cm2. Three mixtures of the two repellents were also evaluated (DEET:IR3535 1:1, 3:1 and 1:3), applied at the above mentionated concentrations. Four independent replicates of each assay were performed. Results were analyzed using one-way ANOVA. DEET and IR3535 showed the same Lowest Observed Effect Level (LOEL): 1.15 µg/cm2. In general, the mixtures showed a repellent effect slightly lower than their pure components, but the vast majority of these differences were not statistically significant. This is the first evaluation of the repellent effect of IR3535 on T. infestans. We will continue looking for interactions between repellents in Chagas disease vectors and other insects, focusing our effort on mixtures containing synthetic and natural compounds.