UNIDEF   23986
UNIDAD DE INVESTIGACION Y DESARROLLO ESTRATEGICO PARA LA DEFENSA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Aedes aegypti larvae respond to Natural and Synthetic Odorants.
Autor/es:
PAULA GONZALEZ; PAOLA GONZALEZ AUDINO; HÉCTOR MASUH
Lugar:
Victoria
Reunión:
Conferencia; International Chemical Ecology Conference (ICEC) 2013; 2013
Institución organizadora:
International Society of Chemical Ecology
Resumen:
The ability to detect a wide range of sensory cues is essential for the survival and vectorial capacity of mosquitoes. This investigation focuses on the analysis of olfactory-driven behavior in dengue vector mosquito Aedes aegypti (L.)  larvae to respond to synthetic or natural odorant stimuli. We established a sensitive olfaction-based behavior assay, following the technique previously established [1]. In this assay, the distribution of 100 2nd or 3nd instar larvae of Ae. aegypti was monitored to a range of odorants stimuli as well as appropriate negative controls throughout a 60 minute time course. The number of larva in both test and control zone was counted throughout all time-point and performance index (PI) at 40min was calculated to represent the response characteristics of each odorant with +1 indicative of full attraction while -1 represents complete repulsion. The widely used insect repellent N, N-diethyl-m-toluamide (DEET), consistently evoked highly significant negative PI as well as Indole and Acetophenone. When using yeast extract, a know food source, the larvae responded with a significantly positive PI as well as 2-Methylphenol, 1-Octen- 3-ol, 3-Meyhylphenol and fish food. We also found that, consistent with the olfactory function, ablation of the larval antennae eliminated these behavioral responses. It is important to study the behavior of attractant and repellency, as it could contribute to the understanding of the mechanism of olfaction in Ae aegypti larvae.     [1] Yuanfeng Xia, et al. (2008). PNAS, 105 (17): 6433- 6438.