UNIDEF   23986
UNIDAD DE INVESTIGACION Y DESARROLLO ESTRATEGICO PARA LA DEFENSA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of volatiles from lemon peel in the viability of Anastrepha fraterculus eggs
Autor/es:
RUIZ MJ; ALZOGARAY RA; ARRIGHI F; ARROYO L; GASTAMINZA G; WILLINK E; BARDÓN A; VERA MT
Lugar:
Huerta Grande, Córdoba
Reunión:
Encuentro; Second Meeting of the Latin American Association of Chemical Ecology; 2012
Institución organizadora:
Asociación Latinoamericana de Ecología Química
Resumen:
The chemical properties of fruits, especially the compounds present in the essential oils glands´ from the peel, are considered the most important resistance mechanism of citrus against fruit flies infestation and impacts on the condition of a given fruit as a larval host. It has been reported for several fruit fly species that eggs and larvae are affected by these compounds. In the South American fruit fly it has been shown that lemon is not a host and chemical resistance has been proposed as the involved mechanisms. We evaluated the effect of volatile compounds of essential oil of lemon, ether extracts from the lemon peel and pure compounds such as limonene and citral in the viability of the fuit fly Anastrepha fraterculus eggs. We also evaluated ether extracts from grapefruit. The eggs were exposed to different concentrations of the extracts or pure compounds in Petri dishes for 24 hours. Then, they were transferred into a clean capsule and evaluated on the fifth day to score the number of hatched and unhatched eggs. Values of Lethal Dose 50% (LD50) were calculated for each extract or pure compound tested. In addition, we chemically characterized the extracts using a Hewlett Packard II 5890 mass gas chromatography (GC-CM). The LD50 for the lemon oil and ether extracts (either lemon or grapefruit) were similar (p > 0.05) and both pure compounds showed to be toxic. The gas chromatography showed that the relative amounts of limonene, the citrus oils major compound, were similar for the lemon oil and the ether extracts. In spite of this, the fruit extracts presented differences in their minor compounds and the extract from grapefruit presented a higher diversity of compounds. We show that the compounds present in the flavedo of the citrus species evaluated are toxic to the eggs of A. fraterculus, confirming the presence of chemical resistance mechanisms.