Carvone modulates in vitro and in vivo the kinetic behaviour and efficacy of abamectin.

MIRÓ, MARÍA VICTORIA; LLOBERAS, M; LANUSSE, CARLOS EDMUNDO; LUQUE, SONIA; CANTÓN, CANDELA; VIRKEL, GUILLERMO LEÓN; CARDOZO, P; COSTA-JUNIOR, LIVIO; LIFSCHITZ, ADRIAN LUIS

Utah

Congreso; The 65th Annual Meeting of the American Association of Veterinary Parasitologists; 2020

The search of novel strategies to control gastrointestinal nematodes in ruminants is aconcern considering the increasing of anthelmintic resistance. Bioactive phytochemicals maycontribute to improve parasite control by enhancing the effect of existing anthelmintic drugs.This work assessed the in-vitro and in-vivo pharmacological interaction and the in-vivoefficacy of abamectin (ABM) combined with the plant-derived compounds carvone (CNE), inlambs naturally infected with resistant gastrointestinal nematodes. At first, the modulation ofP-glycoprotein (P-gp) by CNE was assessed using the intestinal explant model. Rhodamine123 (Rho123) and ABM were used as substrates to measure their accumulation in cattleileum in presence or absence of CNE. For the in vivo assay, twenty-eight (28) lambs wereallocated into three (3) experimental groups. Each group was treated orally with either ABM(0.2 mg/kg), ABM in combination with CNE (100 mg/kg, four doses every 24 h) or remainedas untreated control. Blood samples were collected between 0 and 168 h post-treatment andplasma levels of both compounds were determined by HPLC. Individual fecal samples werecollected on days -1 and 14 post-treatment to perform the fecal eggs count reduction test.The presence of CNE increased significantly (P