Evaluation of the immunogenicity of the recombinant protein GSTMu of Fasciola hepatica (rFhGSTMu) adsorbed on aluminum hydroxide in sheep

FERNANDEZ VANESA; GUTIÉRREZ, SILVINA E; SOLA JAVIER; ESTEIN, SILVIA M; MORAN CELESTE

Mar Del Plata

Jornada; Reunión Anual de SOCIEDADES DE BIOCIENCIA SAIC . SAFE . SAB . SAP; 2019

Fasciola hepatica is a zoonosis which causes significant economic losses in ruminants. The development of a vaccine emerges as an alternative for the control of this disease. The recombinant protein GST Mu from F. hepatica (rFhGSTMu) adsorbed on aluminum hydroxide (rGSTMu + Al (OH)3) conferred 90% protection against this parasite in the mouse model. The objective of this work was to evaluate the humoral and cellular immune response against this vaccine in the susceptible species. Corriedale female sheeps were immunized twice every 30 days. The humoral immune response was analyzed byan indirect ELISA. Blood was obtained every 15 days from day 0 to 75. The in vivo immune cell response was evaluated on the day 75 by performing the intradermal test with rFhGSTMu.Specific serum IgG antibodies increased after each boost unlike that observed in the control group (without immunization), which maintained baseline levels throughout the trial. The differences were maximum and significative (p