METABOLIC STABILITY OF GLIFOSATE IN RUMINAL CONTENT FROM CATTLE: PARTITION BETWEEN PARTICULATE AND FLUID DIGESTIVE MATERIAL

PERÉZ, VANINA; LARSEN, KAREN E.; VIRKEL, GUILLERMO L.; LIFSCHITZ, ADRIÁN L.

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencia; 2019

Glyphosate (GLP) is one of the most commonly pesticides delivered to the environment. Farm animals could be exposed to both GLP and aminomethylphosphonic acid (AMPA, the major GLP metabolite) present in surface and groundwater, but also in foodstuffs used for preparation of concentrate feeds. For instance, detectable levels of GLP were observed in the urine of dairy cows chronically exposed to the herbicide present in their feed. In cattle, the rumen plays a central role in the pre-systemic metabolism of xenobiotics, thus protecting the organism against potentially harmful chemical compounds. This work evaluated the chemical stability of GLP in the ruminal environment. Ruminal contents from 3 steers were collected in a local abattoir. Samples were roughly filtered, kept at 37°C and transported to the laboratory. Aliquots of both whole ruminal content and fluid phase were incubated (3-6 h) in anaerobiosis with GLP (12.5 µg/mL). Metabolic viability of ruminal contents was assessed by the measurement of the SO-reduction of the anthelmintic albendazole sulfoxide (ABZSO). These assays were carried out in the absence (controls) and in presence of GLP. Incubations of boiled (inactive) ruminal content and fluid were used as controls. Samples were analysed by HLPC with fluorescence detection. Incubated ruminal contents were subsequentially centrifuged for determination of the amounts of the herbicide in the fluid phase and in the particulate material. GLP was not metabolised neither in the ruminal content nor in the fluid phase. The percentage of GLP associated to the particulate material ranged between 5.7 (3 h) and 11.2 (6 h). GLP did not affect the SO-reduction of ABZSO. These results may indicate that the ruminal environment is not involved in the pre-systemic metabolism of GLP. As GLP is a hydrophilic xenobiotic, the highest proportion of the herbicide is solubilised in the fluid phase of the ruminal content which may influence in its rate of absorption after ingestion.