CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ANTIMICROBIAL RESISTANCE IN PIGS AND SWINE FARMS: A CAUSE FOR CONCERN AND A SOURCE OF GENE TRANSMISSION RESISTANCE
Autor/es:
PÉREZ GAUDIO D.S.; COLELLO R.; FERNÁNDEZ D.; MOZO J; MARTÍNEZ G.; FERNÁNDEZ PAGGI M.B.; DECUNDO J.M.; ROMANELLI A.; DIEGUEZ S.; ETCHEVERRÍA A.; PADOLA N.L.; SORACI A.L.
Lugar:
Sveti Martin na Muri
Reunión:
Congreso; European Symposium on Antimicrobials and Antimicrobial Resistance - CESAR 2018; 2018
Institución organizadora:
International Centre for Genetic Engineering and Biotechnology Trieste
Resumen:
The spread of antimicrobial resistance (AMR) in bacteria derived from food-producing animals is a consequence of the intensive veterinary use of antibiotics (ATB). Integrons are involved in the acquisition of AMR. They have an intI gene encoding an integrase (intI1or intI2). The objectives of this work were a-) to investigate the presence of intI in commensal E. coli strains isolated from pigs and farm environment; b-) to evaluate the ATB susceptibility of commensal isolated integron (+) E. coli, and c-) to determine the horizontal transference of intI1 from a (+) isolated E. coli strain to a (-) STEC 0157:H7 strain. 5 postpartum sows and 5 piglets from each litter were sampled by rectal swabs at days 0, 21 and 70 after birth. Farm environmental samples were also obtained. Susceptibility of isolates to different ATB was tested (Kirby Bauer disk diffusion method). To determine the horizontal transference of intI1 between a resistant and a sensitive strain a conjugation assay was achieved. PCR were performed to detect intI. Percentages showed an increase along the production system. E. coli carrying integrases were detected in 100% of the sows. From isolates recovered from piglets, 49.4% of resistant strains carried intI1, while 42.9% carried intI2 and 7.7% carried both. In piglets, an 88.3% of the strains were multiresistant, while a 27.3% of sows? isolates were resistant to more than 2 ATB. 78.4% of the E. coli isolated from the environment contained an intI. Most of the isolates were resistant to 1or 2 ATB. The conjugation assay showed that at 4 h, 67% of the initially intI1 negative and Shiga-toxin producing E. coli colonies acquired the intI1 gene. In conclusion, it was demonstrated that commensal E. coli isolated from sows harbored the genes for IntI. It was also confirmed the presence of integrons in strains isolated from piglets younger than 12 h of age, suggesting the importance of the transmission of resistant strains at birth. It was proved the AMR transfer from an E. coli strain isolated from the farm to a negative and probably pathogenic Shiga toxin-producing strain, in a period of only 4 h. The abundance of commensal E. coli harboring integrons reported in this study is alarming and suggests that unmonitored use of ATB in pig farms could expand the abundance of AMR reservoirs in the environment, and also impacts on public health, due to the risk of transfer by direct contact of farm and food industries workers and/or by the food chain.