CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MOLECULAR GENETIC VARIABILITY DIVERSITY AND RELATEDNESS OF FASCIOLA HEPATICA ISOLATES FROM DIFFERENT HOSTS AND GEOGRAPHIC REGIONS USING THE RAPD-PCR TECHNIQUE
Autor/es:
SCARCELLA SILVANA; COSSIO-BAYUGAR RAQUEL; GISELA NEIRA; SOLANA HUGO; MIRANDA-MIRANDA ESTEFAN; ROBERTO MERA Y SIERRA
Lugar:
Santiago de Chile
Reunión:
Congreso; FLAP XXIV Congreso Latinoamericano de Parasitología; 2017
Institución organizadora:
Sociedad Chilena de Parasitología
Resumen:
Introduction: Fascioliasis is an important parasitic disease of domestic animals and humans worldwide,causing large economic losses to the livestock industry and producing ever-increasing public health awarenessas a neglected zoonotic parasitosis.Studies regarding the genetic constitution as well as status of geneticvariation of the parasite populations have significant implications on the epidemiology and successful designof campaigns destined for the control of fasciolasis.Objective: The present communication focuses on molecular characterization of F. hepatica isolates usingrandom-amplified polymorphic DNA polymerase chain reaction (RAPDs- PCR) derived from sheep, cattleand buffalo, collected between 2013 and 2016 in different regions of Argentina.Materials and methods: Adult flukes were collected from different hosts from abattoirs in diverse regions ofArgentina. The genomic DNA of each sample was isolated following the standard phenol?chloroformprocedure. Duplicate PCR reactions on each individual template DNA were performed to test thereproducibility of the individual DNA bands. DNA was resolved by agarose gel electrophoresis. The size ofthe fragments was determined by the Rf. Digital processing of amplicon bands was done using Labworks 4.0gel image analysis software. Genetic distances between genotypes were calculated on the basis of Pearson?scoefficient algorithm for dendrogram construction.Results and conclusions: The dendrogram obtained contained two major groups at 50,047 and 50,613 whichclearly diverged from the RAPDS markers within the genome of the parasites, but showed no correlation withrespect to the origin of the host, except for samples obtained from the same host species with a Geneticdistance of 16 or less. Our results suggest that F. hepatica have different patterns of population geneticstructure, which is consistent with a parasite maintaining similar infectivity across several host species withlittle or no importance of the infective-stage host origin, results also suggest that genetic variability dependmore of the different geographical regions than of the definitive hosts.