CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Fenbendazole and triclabendazole effects on CYP1A1/1A2 and FMO1/3 mRNAs in cattle liver slices: preliminary results ina,*,§, Laura Matéb,§, Roberta Tolosia, Paula Viviani, Adrian Lifschitzb, Carlos Lanusseb, Mauro Dacastoa and Guillermo Virkelb
Autor/es:
TOLOSI, R.; LANUSSE, CARLOS E.; MATÉ, M. LAURA; LIFSCHITZ, ADRIAN L.; VIRKEL, GUILLERMO L.; GIANTIN, M.; VIVIANI, PAULA; DACASTO, MAURO
Lugar:
Duseldorf
Reunión:
Conferencia; 20 th International Conference on CYP450; 2017
Resumen:
Background. Fenbendazole (FBZ) and triclabendazole (TBZ) are benzimidazole drugs (BZDs) widely used in veterinary practice as anthelmintics. Members of cytochrome P450 (CYP) and flavin monooxygenase (FMO) superfamilies of drug metabolizing enzymes are primarily responsible of their biotransformation1. The xenobiotic-dependent up-regulation of CYPs is well documented, while FMOs are generally considered not inducible2. In the present study, the effect of FBZ and TBZ (alone or in combination) on CYP1A1/2 and FMO1/3 mRNA levels were measured on precision-cut bovine liver slices (bLS).Methods. Precision-cut bLS from 6 male cattle were obtained according to Maté et al3. Following the fine-tuning of an absolute quantification protocol for target genes4, bLS were incubated for 0, 6 and 12 h with FBZ and TBZ (50 µM), alone or in combination. β-naphthoflavone (βNF, 25 µM) was used as positive control to confirm gene induction. Total RNA was extracted, reverse transcribed and used as template for the measurement of target genes expression (qPCR). The TATA Box binding protein and ribosomal protein lateral stalk subunit P0/β-actin were used as internal control genes to normalize FBZ/TBZ and βNF data, respectively.Results. At T0, CYP1A2 mRNA levels were 25-fold higher than CYP1A1; however, FMO1 and 3 were equally represented. βNF up-regulated CYP1A1 (P