Three-week post-vaccination efficacy of the CSF CL strain produced on ovine cell line against a virulent Classical Swine Fever (Hog cholera)

PEREZ, S. E; RISATTI, G

Chongqing

Congreso; 25th International Pig Veterinary Society Congress; 2018

International Pig Veterinary Society Congress

IntroductionClassical swine fever (CSF) is a highly contagious viral disease classified as a notifiable pig disease by OIE. In an infected environment, vaccines are the basic tools for control and eradication of CSFV. This study aimed at assessing the efficacy of an attenuated CSF CL strain produced on ovine cells against a virulent CSF challenge performed three weeks post-vaccination. Material and methodsTwo groups of 8 CSF-negative crossbred pigs weighing 18 kg were either vaccinated with a live one dose of CL strain (>100 PD50/dose) or left unvaccinated. Three weeks post-vaccination, they were challenged with 5.5log10TCID50 CSFV of Haiti-96 strain both intramuscularly and intranasally with separated aliquots. Clinical signs, rectal temperature were monitored for 28 days post-challenge (DPC) and necropsied. Blood samples, nasal swabs and tonsil scrapings were regularly collected and assayed for blood formulation, sera antibody titres (E2-Erms ELISAs, SN titrations), and viral loads in total blood, nasal and tonsil mucus. ResultsFollowing 5-6 incubation days, all controls showed typical acute CSF. Two of them were euthanized on ethical ground. They also developed severe leukopenia and lymphopenia. Necropsic lesions were evocative of chronic form of CSF. None of the vaccinates developed any sign of CSF.In controls, CSFV viremia was detected from 5DPC reaching levels above 6log10TCID50/mL from 6DPCH to 11DPCH. Tonsils were positive for CSF as well from 5DPCH and viral sheddind in nasal mucus could reach more than 4log10TCID50/mL. Vaccinates showed drastically (p