Quantification of plasma progesterone (P4) in cattle using the ELFA technique

RODRÍGUEZ PÉRSICO, J.M.; BO, G.; TAPIA C.; RAGGIO, S.; ANDUAGA MARCHETTI, I.; BIANCHI, C.; BELTRAMO, M.

Córdoba

Simposio; 12° Simposio Internacional de Reproducción Animal; 2017

Instituto de Reproducción Animal Córdoba

The quantification of hormones involved in the bovine reproductive cycle is important for assessing and diagnosing the reproductive status of the animal. Different clinical interpretations can be done with the determination of progesterone (P4) levels, and values below 1 ng/ml indicate absence of corpus luteum. Furthermore, recent publications have shown that values below 2,7 ng/ml on day 7 or 5 ng/ml on day 14 of the cycle could predict pregnancy loss. Currently, the gold standard technique to measure circulating P4 levels isradioimmunoassay (RIA). However, simpler and more accessible diagnostic techniques are now emerging. The purpose of this study is to evaluate a new commercial in vitro diagnostic assay of P4 based on enzyme immunoassay by competition with detection of final fluorescence (ELFA). A total of 67 blood samples, from 25 cows, were collected using BD Vacutainer (BD, USA) with sodium heparin by jugular venipuncture at different days of estrus cycle, and centrifuged at 3000X g for 30 min for plasma separation, which were frozenat -20 °C until analysis. P4 levels were measured in duplicate for both techniques: IM1188-PROGESTERONE-RIA (Beckman Coulter, USA) and VIDAS-PRG-ELFA (Biomerieux,France). P4 levels obtained by RIA were classified in two groups as A) P4