CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Antiproliferative Effects of Oxytocin and Desmopressin on Canine Mammary Cancer Cells
Autor/es:
BIANCHI, C.P.; BENAVENTE, M.A.; ABA, M.A.; IMPERIALE, F.
Lugar:
Mar del Plata
Reunión:
Otro; XLVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE); 2016
Resumen:
Neoplasms of the mammary gland represent the most frequent tumor type in the female dog, and according to the histologic criteria, approximately 50% are malignant. In the most aggressive cases of mammary cancer, surgery is not enough to warrant a favorable outcome, and adjuvant therapies are needed to improve the patient?s overall survival. Several in vitro studies performed on murine and human cancer cells have suggested that some peptide hormones can modulate tumor growth. However, little is known about its effects on canine cancer. The aim of the present study was to evaluate the effects of Oxytocin (OT) and Desmopressin (DDAVP) on proliferation of the canine mammary cancer cell line CMT-U27. The cells were grown in 96-well plates, at two densities (4x103 and 8x103 cells/well) in 200 µl of RPMI-1640 supplemented with 10% Fetal Bovine Serum. After overnight culture, the medium was removed and replaced with medium containing OT or DDAVP at five concentrations: 10, 50, 100, 500 and 1000 nM, or medium without drugs (controls). After 72 h of incubation, cell viability was determined using the MTT colorimetric dye reduction method. With 4x103 cells/well, OT at 1000 nM resulted in a 25% of inhibition of cell viability (p