TLymphocytes characterization in tuberculin reactor cattle transiting early peripartum period.

TRAVERSA M.J.; SARACCO M.O.; ESTEIN S.M.; PAOLICCHI F.A.; JORGE M.C.

Buenos Aires

Congreso; IV LASID Meeting LXIII Argentinean Immunology Society Meeting.II French-Argentinean Immunology Meeting.; 2015

SAI-French Society of Immunology

transiting early peripartum periodMaría Julia Traversa1, Mónica Olga Saracco2, Silvia Marcela Estein3, Fernando Alberto Paolicchi4, María Cristina Jorge11 Departamento de Sanidad Animal y Medicina Preventiva. Facultad de Ciencias Veterinarias. Universidad Nacional del Centro de la Provincia de Buenos Aires2 Comisión Nacional de Referencia para el SIDA. Facultad de Medicina. Universidad de Buenos Aires3 Centro de Investigación Veterinaria Tandil. Consejo Nacional de Investigaciones Científicas y Técnicas4 Estación Experimental Agropecuaria Balcarce. Instituto Nacional de Tecnología AgropecuariaAbstract: Bovine tuberculosis is a zoonotic chronic granulomatous infectious disease that occurs in bovines and is caused by the intracellular pathogen Mycobacterium bovis. Cell mediated immunity with a T-helper 1 (Th1) bias is of great importance in the control of M. bovis and γδ-Tlymphocytes induce a Th1bias of αβ-Tlymphocytes in tubercoulous cattle. During early peripartum period (EPP) cattle also present an immunesupression. The objective of this job was to characterize γδ-Tlymphocytes in tuberculin reactor dairy cows during EPP. Peripheral blood mononuclear cells (PBMC) were obtained by density gradient centrifugation from noncoagulated blood from 9 Argentinean-Holstein cows older than 2 years, five were tuberculin-positive and transited EPP and four were tuberculin-negative and did not transit EPP. PBMC were indirectly immunolabeled with monoclonal antibodies in cocktail (IgG1/IgM) to detect LTCD4, LTCD8, γδ-Tlymphocytes and CD25 and after that with a cocktail 1:200 antiIgG1PE/antiIgMFITC, fixed and stored in dark until acquisition. 10000 events were acquired with a FACS-CANTO flow cytometer (Becton-Dickinson) and analyzed with FCSExpress software. In tuberculin reactor transiting EPP group it was observed a LTCD4:LTCD8 ratio of 0.95, 11.71% of γδ-Tlymphocytes and a 5.45% of CD25 in control group values were 1.58, 13.37% and 13.10%, respectively. Even though we did not perform dual labelling of γδ-Tlymphocytes to detect CD25 in this subset and differences in CD25 percentages were not statistically significant it is important to notice that in tuberculin-positive cattle transiting EPP the Th1bias was lower and a half of the cells expressed CD25, a constitutive marker of bovine γδ-Tlymphocytes.