Humoral immune response in ewes and maternal passive transfer after immunization with polymeric BLSOmp31 formulated with a nanostructure formed by self-assembly of 6- O-ascorbyl palmitate (CoA)

DÍAZ A.G; SANCHEZ VALLECICO M.F.; PARDO R.; MALETTO B.; PALMA S.; ALLEMANDI D.; GOLDBAUM F.A.; ESTEIN S.M.

Buenos Aires

Congreso; IV LASID Meeting LXIII Argentinean Immunology Society Meeting.II French-Argentinean Immunology Meeting.; 2015

SAI-French Society of Immunology

PS271Humoral immune response in ewes and maternal passive transfer after immunization with polymeric BLSOmp31 formulated with a nanostructure formed by self-assembly of 6-O-ascorbyl palmitate (CoAlejandra Diaz1, María Fernanda Sanchez Vallecillo2, Romina Pardo3, Belkys Maletto2, Santiago Palma4, Daniel Allemandi4, Fernado Goldbaum5, Silvia Estein11 Laboratorio de Inmunología, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), CIVETAN-CONICET, Facultad de Ciencias Veterinarias, UNCPBA, Tandil, Argentina2 Departamento de Bioquímica Clínica, CIBICI (CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina3 Inmunova S.A., Bs. As., Argentina4 Departamento de Farmacia, UNITEFA (CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina5 Fundación Instituto Leloir, Bs. As., ArgentinaAbstract: Brucella ovis causes reproductive failure in sheep with significant economic losses. The only available vaccine is an attenuated strain which shows important disadvantages. Consequently, there is a need for developing of safe and efficient brucellosis vaccines. Previous studies demonstrate that polymeric BLSOmp31 was immunogenic and conferred protection against B. ovis infection in rams. The aim of this study was to analyze the humoral immune response induced in pregnant ewes in serum, secretions and milk after immunization with BLSOmp31 using Coa-ASC16 as vaccine platform.Twelve pregnant ewes were randomly distributed in two groups immunized with BLSOmp31/Coa-ASC16 (0.5 mg/animal) by subcutaneous (n=5) (G1) or by conjunctival route (n=5) (G2) twice (days 0 and 21). Non immunized group was included (n=2) (G3). Samples of sera, saliva, lacrimal, nasal and vaginalSAI 201541secretions were obtained periodically until day 80. After parturition samples of milk were obtained and lambs were bled for obtaining sera. All samples were analyzed by ELISA anti-BLSOmp31 to determine specific IgG antibodies.The immunization of ewes with BLSOmp31/Coa-ASC16 by subcutaneous route induced higher levels of antibodies in serum than conjunctival administration until day 60 while both of them were similar at day 80. No levels of IgG antibodies were detected in any secretion. Milk samples and sera from lambs showed significant levels of IgG antibodies which were higher in ewes immunized by subcutaneous route.These results suggest that vaccination of ewes with BLSOmp31/Coa-ASC16 offers an alternative to induce passive immunity which could help to prevent B. ovis infection in lambs.