Deoxynivalenol induces apoptosis in cell cultures and the antibiotic fosfomycin protects cells from nuclear changes.

PÉREZ, D.S; MARTÍNEZ, G.; RENSETTI, D.E; FERNÁNDEZ PAGGI, M.B; RICCIO, M.B; PÉREZ, S.E; TAPIA, M.O; SORACI, A.L

Florida

Congreso; Global Veterinary Summit; 2015

Global Veterinary Summit

Deoxynivalenol (DON) is a mycotoxin which inhibits protein synthesis and causes cytotoxicity. Fosfomycin (FOS) is a bactericidalbroad-spectrum antibiotic used in human and veterinary medicine with some extra antimicrobial properties. We havedemonstrated that after 4 hours of incubation, FOS was able to prevent the cytopathic effect of DON on Hep2 cells. To corroboratethese results and to determine whether the effect of DON was due to the induction of apoptosis, Hep2 cells were seeded in 6 well-platesand after 24 hours, cell monolayers were treated with DON (2.8 μg/mL) alone or with DON (2.8 μg/mL) and FOS (550 μg/mL). Thepresence of nuclear morphological changes representative of apoptosis was evaluated by DAPI staining under immunofluorescencemicroscope, the percentage of apoptotic cells was determined and the results were evaluated by ANOVA/Tukey test. Cell culturesincubated with DON and FOS were similar to control wells showing the absence of cytotoxicity. Apoptotic cells percentage wassignificantly higher (5.64+0.27) (p