The T687G SNP in a P- glycoprotein gene of F. hepatica is not detected in different strains susceptible and resistant to triclabendazole

SOLANA M.V.; SOLANA H.; ORTIZ OBLITAS P.; MIRANDA-MIRANDA E.; TORT J.

Philadelphia

Conferencia; International Conference on Parasitology; 2015

OMICS International & Journal of Bacteriology & Parasitology

Fasciolosis is a parasitic disease caused by the trematode Fasciola hepatica. Its control is mainly based on the use of triclabendazole (TCBZ), a halogenated benzimidazole with excellent efficacy against juvenile and adult stages. Resistance to TCBZ in F. hepatica has been reported in many parts of the world. Benzimidazole resistance in nematodes is mainly caused by specific amino acid substitutions in β-tubulin resulting from a nucleotide substitution in the gene. However, the resistance to TCBZ in F. hepatica is not associated with changes in this molecule. The increased cellular efflux of TCBZ in F. hepatica is related to ABC transporters as the P-glycoproteins (PGP). In this protein single nucleotides polymorphisms (SNPs) were identified, such as T687G which was associated with the phenomenon of resistance (Wilkinson et al, 2012). Other SNPs not yet associated with resistance were also identified (A617T and T267G).