Morphometrical study of Closantel treated Fasciola hepatica eggs

SOLANA M.V.; MERA Y SIERRA, R.; NEIRA, G; SCARCELLA S.; SOLANA H.

Philadelphia

Conferencia; International Conference on Parasitology; 2015

OMICS International

The trematode Fasciola hepatica is spread worldwide causing important losses in livestock and is also an expanding zoonotic disease. Chemotherapy is fundamental for its control, yet triclabenzadol resistance is appearing, thus closantel can be a valid alternative. It is effective against mature and immature stages yet it does not exhibit ovicidal activity in vitro and the ovicidal effects in vivo at present are undetermined. The Egg Hatch Assay is a valid test to evaluate ovicidal activity and was implemented to evaluate the effect of closantel; morphometrical study was included as part of the characterization of the treated eggs. Sheep were inoculated with Cullompton strain of F. hepatica; eggs were recovered from the gall bladder of control and closantel treated (subcutaneously and orally) at 12, 24, and 36 hours post treatment. Measures were taken on 50 eggs from each were found in length; size; width and shape. No statistically significant differences were found in length and size between control and experimental groups, for another hand significant differences were detected in width and shape. Eggs exposed to Closantel tend to be more elliptical. It is worth highlighting that al the length, width and size of the control eggs are considerably greater than previous descriptions for normal F. hepatica eggs in sheep and other domestic and wild species. This could be a characteristic of the Cullompton strain, yet studies should be done to assess if these large eggs are isolated strains or there is greater variability in egg morphology than previously supposed