SIMULTANEOUS IMMUNIZATION OF CATTLE WITH FOOT-AND-MOUTH DISEASE (FMD) AND LIVE ANTHRAX VACCINES. , M. Trotta; O. Melucci ; M. Catena; F. Fernández and A. Capozzo

LAHORE, J.; TROTTA, M; MARÍA CATENA

Cavtat,

Congreso; Where science and policy meet: FMD RISK MANAGEMENT in a world of changing disease landscapes; 2014

European Commission for the Control of Foot-and-Mouth Disease

SIMULTANEOUS IMMUNIZATION OF CATTLE WITH FOOT-AND-MOUTH DISEASE (FMD) AND LIVE ANTHRAX VACCINES. Lahore1#, M. Trotta2#; O. Melucci1 ; M. Catena1; F. Fernández2 and A. Capozzo2* 1 Facultad de Ciencias Veterinarias. Universidad del Centro. Tandil. Buenos Aires, Argentina, 2 INTA, Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas. Buenos Aires, Argentina # Both authors equally contributed to this work * Presenting author Introduction: FMD-vaccination in Argentina is performed under the control of the regulatory authorities and conducted by certified veterinarians. These organized campaigns may facilitate the controlled application of other vaccines against other endemic diseases, like Anthrax, which is controlled with a live vaccine. There is no information on the interference of immunity against FMD vaccines when applied together with a live bacterial vaccine. In this study we evaluated if the simultaneous application of Bacillus anthraces vaccine with the commercial tetravalent oil-based FMD vaccine used in Argentina modifies the antibody booster responses against FMDV. Materials and methods: Two groups of 15 bovines each; with comparable Liquid Phase Blocking ELISA (LPBE) titers at 0 days post vaccination (dpv) were immunized with the currently used tetravalent FMDV vaccine (FMD-V) and a commercial attenuated bacterial vaccine (ABV) containing not encapsulated non-virulent spores (Sterne strain of Bacillus anthraces F234; 18,000,000 spores per dose). Both vaccines were applied simultaneously in different sides of the neck. Serum samples were obtained at 0, 25, 60 and 90 dpv and antibody titers against the vaccine O1/Campos strain were measured by LPBE and isotype-ELISA. Results: Bovines immunized with FMDV-V or FMDV-V and ABV responded with a boost in the LPBE antibody titers at 25 dpv and remained within similar levels up to 90 dpv. However, animals vaccinated with FMD-V had significantly lower LPBE titers at 25 and 90 dpv, compared to those immunized simultaneously with both vaccines. The difference in total Ab titers at 25 dpv was due to an increase in IgG2 titers. Overall, kinetics of antibody titers was comparable. Conclusions: The combined application of a live vaccine with the current FMD tetravalent vaccine used in Argentina did not interfere with the antibody FMDV-booster responses. Antibody titers were similar in both groups and followed comparable kinetics over time.