CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cellular immune response in dairy cattle naturally infected with fasciola hepatica in Cajamarca, Peru
Autor/es:
SILVA DIAZ H.; HOBÁN VERGARA C.; SOLANA H.; CABRERA M.; ORTIZ OBLITAS P.
Lugar:
Mexico DF
Reunión:
Congreso; 13th International Congress of Parasitology ICOPA XIII; 2014
Institución organizadora:
The Mexican Society of Parasitology and the World Federation of Parasitologists The Mexican Society of Parasitology and the World Federation of Parasitologists
Resumen:
BACKGROUND: Fasciolosis produced by Fasciola hepatica is an important parasitic diseaseof livestock in many countries. In Cajamarca, Peru, prevalence rates above 80% are reportedin dairy cattle. The cellular immune responses is poorly understood in natural infection, hencethe present work studied the proliferative capacity and expression of cytokines (IFN-γ and IL4)in peripheral blood mononuclear cells (PBMC) from cows and calves against nonspecificPhytohemagglutinin (PHA) and specific Excretory/Secretory antigens from immature (FhE/S-I)and mature stages (FhE/S-M) of F. hepatica. METHODS: The proliferative capacity was determined by in vitro lymphocyte proliferation, andcytokine expression was evaluated in cell culture supernatants by ELISA.RESULTS: Cows infected with F. hepatica showed a decreased capacity to respond againstnonspecific and specific proliferative stimulus. When the cytokine profile was studied, IFN-γ expression was low with high IL-4 against specific stimulus indicating that the responseremains polarized towards a TH2 type response. Naturally infected calves expressed higherIFN-γ levels compared to cows, with a similar expression of IL-4 between both groups ofanimals. These results demonstrate an immunomodulatory response in adult animals as thedisease progresses. No differences were observed regards the immune response to antigensof immature and mature stages of the parasite.CONCLUSIONS: Our results suggest that cows infected with F. hepatica respond with apolarized TH2 immune response with low levels of IFN-γ and a high IL-4 response. On theother hand, infected calves expressed significantly higher levels of IFN-γ compared with cows,but both groups expressed similar quantities of IL-4.!