CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Developmental stage-induced carboxylesterase isozymes in the parasitic trematode Fasciola hepatica.
Autor/es:
MIRANDA-MIRANDA E.; COSSÍO-BAYÚGAR R.; PEDROZA-GÓMEZ Y. ; PÉREZ-DE LA ROSA J. ; SCARCELLA S.; SOLANA S.
Lugar:
Mexico DF
Reunión:
Congreso; 13th International Congress of Parasitology ICOPA XIII; 2014
Institución organizadora:
The Mexican Society of Parasitology and the World Federation of Parasitologists The Mexican Society of Parasitology and the World Federation of Parasitologists
Resumen:
BACKGROUND: Steroid hormone-like molecules exert control on developmental changes of helminthes into fully reproductive adults. All invertebrates exhibit a very delicate balance of circulating steroids and the ubiquitous enzymes carboxylesterases are necessary for hydrolyzing excess hormone levels or completely depleting it for the final transition into adulthood. The liver fluke Fasciola hepatica exhibit abundance of carboxylesterase activity already reported in the literature, however very little scientific information is available on this enzyme role on controlling the parasite?s development. METHODS: Closed cycle laboratory-cultured Fasciola hepatica anthelminticsusceptible reference strain was used as the source of carboxylesterases. In vitro excysed metacercariae, parenchyma migratory larvae and adult developmental stages were analyzed by PAGE carboxylesterase zymograms.RESULTS: A 90 kDa. band containing most of the enzyme activity (2500-6000 enzymeunits/mg of protein by Gomori?s assay) was found in all developmental stages underdenaturing SDS-PAGE, however when the proteins were sorted by charge under nondenaturingPAGE, the 90 kDa band split into seven different isozymes each exhibiting  a different electrophoretic retention factor (Rf), which combined showed a distinctive zymogram pattern for each developmental stage.CONCLUSIONS: Our results suggest that F. hepatica controls its developmental molting process by the catalytic action of seven different 90 kDa carboxylesterases isozymes. Our study also  Suggests that controlling the action of different carboxylesterases isozymes may tamper the liver fluke?s biological cycle.