CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of uterine bacterial inflammation in donor mare plasmatic progesterone (PP4) at flushing time. Is effluent analysis a helpful tool?
Autor/es:
FUMUSO E; MARINONE AI; BRUNO S; BRUST A.; HERRERA MF; REDOLATTI, C; BIANCHI C; LOSINNO L
Lugar:
Vancouver
Reunión:
Simposio; 8th International Symposium of equine embryo transfer; 2012
Resumen:
Our objectives were to analyze the impact of uterine inflammation on PP4 at the time of embryo recovery and evaluate if exfoliative cytology (EC) and bacterial culture (BC) effluents from the embryo evaluation plate could be a useful tool in an Equine Embryo Transfer Program. This study was performed using Silla Argentino mares in Tandil, Argentina, between November 2011 and February , 2012. Eighty six plasma samples and effluents were analyzed from donor 20 mares. Blood samples were taken from the jugular vein and immediately centrifuged. Separated plasma was stored at -20 °C until analysis. Concentrations of P4 were analyzed by a RIA kit (Diagnostic Products Corporation, Los Angeles, CA, USA). Assay sensitivity of P4 was 0.1 ng ml-1 and the intra-assay coefficient of variation was less than 13% for concentrations between 0.1 and 40 ng ml-1. Mares previously inseminated were flushed on day 7 or 8 post-ovulation and samples from the embryo evaluation plate were taken under laminar flow hood, and centrifuged. The sediment was used for analysis. Sterile swabs were used to prepare slides for cytology and a quick stain was used to identify Polymorphonuclear (PMN) cells in 10 fields at 1000X. An average >4 PMN’s were considered positive. Another swab was used for bacteriology seeded in blood agar, and incubated for 48 h at 37ºC. More than 5 colonies was considered positive. Bacteria were classified as gram negative (G-) or positive (G+) and typified. Criteria for embryo, cytology and culture were negative or positive. Comparisons of PP4 values were made from flushings showing: EC&BC neg, EC&BC pos to G-, EC&BC positive to G+, and EC negative & BC +. Data were analyzed using the GraphPad Instat software support, Kruskal-wallis ANOVA test analysis was performed and the value obtained was p0.05). G- bacteria were Pseudomonas aeruginosa (n: 9) and Escherichia coli (n: 1) and G+ were Streptococcus zooepidemicus (n: 5) and Staphylococcus aureus (n: 1). We conclude on this preliminary trial that plasmatic P4 showed different patterns according bacterial strain and effluent samples showed to be useful at the time to decide if treatment in a donor mare should be necessary.