Comparative histomorphological study of endometrium in mares

CANTATORE, S.; HERRERA, J. M.; FELIPE, A.; FUMUSO, E.; HERRERA, M.; AGUILAR, J.

ANATOMIA HISTOLOGIA EMBRYOLOGIA-JOURNAL OF VETERINARY MEDICINE SERIES C

WILEY

Año: 2018 p. 1 - 6

0340-2096

Uterine acute postbreeding inflammation is a physiological tissue response to the entry of exogenous elements, being persistent endometritis the main pathology responsible for subfertility in the mare (Equus ferus caballus; Linnaeus, 1758). Mares can be classified as susceptible or resistant to endometritis according to their ability to remove intrauterine fluid within 48 hours after experimental inoculation. Endometrial biopsy is a technique that is commonly used to establish the degree of lesions that can affect the fertility of the mare. Endometrial histomorphometry is an objective and highly precise diagnostic method. The aim of the present study was to compare, during oestrus, the endometrial histomorphometry of mares previously classified as susceptible or resistant to endometritis. Endometrial biopsies from 24 mares at the oestrus phase of the cycle were obtained. For the histomorphometric analysis, samples were histologically processed and subjected to routine Hematoxylin-Eosin staining. For the evaluation, the following variables were considered: 1-Height of the lining and glandular epithelia (Lining SM=15.9 μm vs. RM=13.3 μm; Glandular SM=15.0 μm vs. RM=13.0 μm); 2-Perpendicular diameters of endometrial glands (SM=51.3 μm vs. RM=44.8 μm); 3-Number of endometrial glands per field (SM=24.8 glands/field vs. RM=20.5 glands/field). The results from this study suggest the existence of a relationship between the studied characteristics and the susceptibility/resistance to endometritis in mares. Thus, increased epithelial height, greater glandular density, and greater development of the glands during oestrus would be related to a higher susceptibility to endometritis.